Useful Experimental Protocols
1. Two-Bottle Test Methods
Barcodes for 30 Water Bottles (requires 3 of 9 font) [Word] [Pages]
Barcodes for 30 Solution Bottles (requires 3 of 9 font) [Word] [Pages]
Doorsheet for Deprivation and Two-Bottle Tests [Excel] [Numbers]
Instructions for Weighing Bottles for Intake Tests [Word]
Barcode 3 of 9 font (FRE3OF9X) [TrueType Font]
Phenol-Chloroform Extraction / Ethanol Precipitation [Word]
Lithium Precipitation [Word]
Transcardiac Perfusion [Word]
Perfusion Buffers [Word] Cheat sheet for mxing up formaldehyde perfusion solution and saline preperfusion solution.
Freezing Sliding Microtome [Word]
Gelatin Subbing of Slides [Word]
Methyl Green Counterstaining [Word]
Cresyl Violet Counterstaining [Word]
Brain Inventory sheet [Word] A table for posting on the fridge door listing all the brains and their progress in getting processed.
Immuno Time sheet [Word] The little sheet we use to record the exact times when the tissue sections were moved from step to step during the immuno.
Immunohistochemistry Buffers [Word] The cheat sheet for mixing up PB, PBS, PBS/BSA, and PBS/BSA/Triton.
Immunohistochemistry Staining [Word]
NADPH Diaphorase Activity [Word] The quick protocol for rapidly staining for presence of nitric oxide synthase enzyme activity.
Oligo Tail-Labeling [Word]
cDNA Random Primed Labeling [Word]
Riboprobe Labeling [Word]
In Situ Hybridization with Free-Floating Sections [Word]
Photoemulsion dipping of In Situ Slides [Word]
Laser Capture Microscopy [Word]
BioRobotics Arrayer [Word]
This page last updated on October 19, 2008 .